Article: Kelly, NH; Schimenti, JC; Ross, FP; van der Meulen, MCH; (2016) “Transcriptional Profiling of Cortical Versus Cancellous Bone from Mechanically-loaded Murine Tibiae Reveals Differential Gene Expression”, Bone, 86: 22-29
Abstract: Mechanical loading is an anabolic stimulus that increases bone mass, and thus a promising method to counteract osteoporosis-related bone loss.
The mechanism of this anabolism remains unclear, and needs to be established for both cortical and cancellous envelopes individually. We hypothesized that cortical and cancellous bone display different gene expression profiles at baseline and in response to mechanical loading.
To test this hypothesis, the left tibiae of 10-week-old female C57B16 mice were subjected to one session of axial tibial compression (9 N, 1200 cycles, 4 Hz riangle waveform) and euthanized 3 and 24 h following loading. The right limb served as the contralateral control. We performed RNA-seq on marrow-free metaphyseal samples from the cortical shell and the cancellous core to determine differential gene expression at baseline (control limb) and in response to load. Differential expression was verified with qPCR. Cortical and cancellous bone exhibited distinctly different transcriptional profiles basally and in response to mechanical loading. More genes were differentially expressed with loading at 24 h with more genes downregulated at 24 h than at 3 h in both tissues. Enhanced Wnt signaling dominated the response in cortical bone at 3 and 24 h, but in cancellous bone only at 3 h. In cancellous bone at 24 h many muscle-related genes were downregulated.
These findings reveal key differences between cortical and cancellous genetic regulation in response to mechanical loading. Future studies at different time points and multiple loading sessions will add to our knowledge of cortical and cancellous mechanotransduction with the potential to identify new targets for mouse genetic knockout studies and drugs to treat osteoporosis. ( C) 2016 Elsevier Inc. All rights reserved.
Funding Acknowledgement: National Institutes of Health [R01-AG028664, R21-AR064034]; NSF GRF
Funding Text: National Institutes of Health Grants R01-AG028664 (MCHM) and R21-AR064034 (MCHM), NSF GRF (NHK).