Wolbachia PCR – High School **

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This kit includes:

• Thermalcycler
• 1 centrifuge (converted for PCR microtubes)
• 42 beaded PCR tubes
• 16 red micropipettors (2-20 µL)
• 4 boxes yellow micropipette tips
• 8 racks for 1.5 mL microtubes
• 8 racks for beaded PCR microtubes (purple and red)
• Sterile water in tube rack (1 tube per team of 2-4 students)
• Empty tube rack to ship PCR samples back to CIBT
• 1 box of 12 ultra fine Sharpies
• Self-addressed FedEx label for shipment of PCR products & DNA extractions on ice
• 1 lab notebook

Sent on ice via FedEx Overnight:

• Wolbachia DNA

• Primers

Teachers must supply:

• 2 DNA samples from morphospecies
• Disposable gloves
• Waste beaker
• Safety goggles (optional)