Abstract 110
Process for production of tilapia retorted skin gelatin hydrolysates with optimized antioxidative properties
Process Biochem. 44 (10): 1152-1157, 2009
Yang, J.-I., Liang, W.-S., Chow, C.-J. and K.J. Siebert
Thermally hydrolyzed tilapia skin gelatin demonstrated noticeable free radical scavenging and inhibition of lipid peroxidation. Five factors in production of retorted skin gelatin hydrolysate (RSGH) were screened using a fractional factorial design to identify critical factors. Phosphoric acid concentration, water/skin ratio, and retorting time had significant effects on α,α-diphenyl-β-picrylhydrazyl (DPPH) scavenging by RSGHs. A face centered, central composite design in these three factors was used to collect data that resulted in strong response surface models of DPPH scavenging (R2 = 0.978) and inhibition of lipid peroxidation (R2 = 0.967). The most effective condition resulted in 80.3% DPPH scavenging and 75.0% inhibition of lipid peroxidation. The models were used to predict maxima for the two properties. These were 79.4% for DPPH scavenging activity and 77.1% for lipid peroxidation inhibition. Antioxidative tilapia RSGH has potential as a natural antioxidant because a large amount of low-priced skin byproducts can be obtained from the tilapia filleting industry.
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