Abstract 023
Yeast Counting by Microscopy or by Electronic Particle Counting
J. Amer. Soc. Brew. Chem. 42: 71-79, 1984
K.J. Siebert and T.J. Wisk
Optimization of operating conditions for the Coulter Counter (electronic particle counting) and the use of several methods for breaking yeast clumps (addition of pepsin and/or EDTA, and different sonicator energy levels and treatment times) are described. Results of a comparison in which samples drawn from brewery fermenters were examined by both the microscopic and Coulter Counter methods showed that early in fermentation the latter produced much lower results, the difference being directly related to the number of yeast cell buds present. Electronic particle counting was much faster and much more precise than microscopic counting.
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