We used a reverse genetics approach to identify residue 802 (lysine, K) of the GFLV strain GHu RNA1-encoded RNA-dependent RNA polymerase (RdRp) as a modulator of vein-clearing symptom development in Nicotiana benthamiana. Mutations to this site abolished (K to G, A,or Q) or attenuated (K to N or P) symptom expression. Noteworthy, residue 802 is necessary but not sufficient for vein clearing, as GFLV-F13 RNA1 carrying K802 remained asymptomatic in N. benthamiana. No correlation was found between symptom expression and RNA1 accumulation, as shown by reverse transcription-quantitative polymerase chain reaction. Structure predictions for the RdRp suggested that residue 802 is flanked by strongly predicted stable secondary structures, including a conserved motif of unknown function (805LLKT/AHLK/RT/ALR814).
-
Recent Posts
- °Predictive modeling of proteins encoded by a plant virus sheds a new light on their structure and inherent multifunctionality
- °Lab members Anna Wunsch and Maddie Flasco defend their theses!
- °Two distinct genotypes of S. festinus reproduce and differentially transmit grapevine red blotch virus
- °Tori Hoyle is awarded a USDA-NIFA Fellowship.
- °Maddie Flasco receives the 2023 Gilmer Award!
- °Distinct Red Blotch Disease Epidemiological Dynamics in Two Nearby Vineyards
- °Brandon is an IES Graduate Research Grant Recipient!
- °Profiling changes in the plant proteome and transcriptome during grapevine fanleaf virus infection
- °Anna is awarded the 2023 Outstanding Service Award!
- °The three-cornered alfalfa hopper is a vector of grapevine red blotch virus in vineyards