Article: Lee, S; O’Dell, D; Hohenstein, J; Colt, S; Mehta, S; Erickson, D; Van Meerbeek, I; Whitehead, J; Sinibaldi, E; Mazzolai, B; Shepherd, RF; “NutriPhone: a mobile platform for low-cost point-of-care quantification of vitamin B-12 concentrations”, Scientific Reports, 6
Abstract: Vitamin B-12 is necessary for formation of red blood cells, DNA synthesis, neural myelination, brain development, and growth. Vitamin B-12 deficiency is often asymptomatic early in its course; however, once it manifests, particularly with neurological symptoms, reversal by dietary changes or supplementation becomes less effective. Access to easy, low cost, and personalized nutritional diagnostics could enable individuals to better understand their own deficiencies as well as track the effects of dietary changes. In this work, we present the NutriPhone, a mobile platform for the analysis of blood vitamin B-12 levels in 15 minutes. The NutriPhone technology comprises of a smartphone accessory, an app, and a competitive-type lateral flow test strip that quantifies vitamin B-12 levels. To achieve the detection of sub-nmol/L physiological levels of vitamin B-12, our assay incorporates an innovative “spacer pad” for increasing the duration of the key competitive binding reaction and uses silver amplification of the initial signal. We demonstrate the efficacy of our NutriPhone system by quantifying physiologically relevant levels of vitamin B-12 and performing human trials where it was used to accurately evaluate blood vitamin B-12 status of 12 participants from just a drop (similar to 40 mu l) of finger prick blood.
Funding Acknowledgement: National Science Foundation [IIP-1430092]; National Science and Engineering Research Council of Canada (NSERC)
Funding Text: D.E. and S.M. acknowledge funding from the National Science Foundation through grant IIP-1430092. In addition, S.L. acknowledges the support of the National Science and Engineering Research Council of Canada (NSERC) through a Postgraduate scholarship. S.L would like to thank Aadhar Jain and Elizabeth Rey for the helpful discussions on the assay development. The authors also thank Erica Bender for performing finger pricks and venipunctures in human trials, and Vicky Simon for running the Immulite system in the Human Metabolic Research Unit in the Division of Nutritional Sciences. The optical density measurement described in this paper was performed in the Nanobiotechnology Center at Cornell University (NBTC).