Identification of mold isolates is done through ITS sequencing followed by alignment with reference isolate sequences.
What is ITS sequencing?
ITS sequencing (internal transcribed spacer) is a method that utilizes spacer DNA that is situated between rRNA genes. This sequence is easily amplified because the sequences that flank the ITS region are highly conserved. Furthermore, ITS regions can have high sequence variability even between closely related species, which can be useful in phylogenetic comparisons.
Fee per sample pricing for ITS sequencing and typing:
Submitted as a pure culture:
- 12 or more isolates: $100/each
- Fewer than 12 isolates: $200/each
Submitted as a mixed culture:
- 12 or more isolates: $150/each
- Fewer than 12 isolates: $300/each
Submitted as a product or swab sample:
- 12 or more isolates: $200/each
- Fewer than 12 isolates: $400/each
Fungal Genome Sequencing Fee Schedule:
Fungal WGS (<15 Mb genomes) – price per genome
- Single Isolate: $2,000
- 2-9 Isolates: $1,000
- 10+ Isolates: $550
Fungal WGS (15-30 Mb genomes) – price per genome
- Single Isolate: $2,250
- 2-9 Isolates: $1,250
- 10+ Isolates: $750
Fungal WGS (30-45 Mb genomes) – price per genome
- Single Isolate: $2,500
- 2-9 Isolates: $1,500
- 10+ Isolates: $1,000
SNP and/or single loci comparison within isolates of the same species (price per analysis): $2,000
Sample packaging and shipping:
Package your sample(s) according to the proper shipping regulations regarding content identity, layers of containment, and of inclusion of dry ice and also overnight your package as well.
Please email your completed isolate submission form and or any questions to: abs276@cornell.edu
Shipping address: 411 Tower Rd, Ithaca, New York 14850 (Courtesy of Dr. Abby Snyder, 343 Stocking Hall)
Requested submission information will be provided or assigned with each isolate or sample:
- Date received (assigned by Cornell)
- ID number (assigned by Cornell)
- Company ID (assigned by company and included in database metadata, if desired)
- General isolation source (finished product, raw material, environment)
- If desired, more specific information can be provided in an open response field
- Plant code or number (blinded, provided by company)
Database Management for Individual Groups
How can we maintain our collection of strains and identify trends in subtyping?
Spoilage yeast and molds can be introduced from a variety of sources. However, without rigorous sampling and subtyping, root cause cannot be determined. Our lab performs various subtyping analyses (ITS and other amplicons as well as whole genome sequencing), as described above. But to leverage these findings, groups often need: (1) a system for documenting findings over time, (2) a method for identifying closely related isolates so that timely investigations can be initiated, and (3) a culture collection to facilitate follow-up investigations. We offer maintenance of collections and databases for groups who submit isolates to our lab for typing. Fees vary based on the request, but as a general estimate:
Research and database development: $5,000
Typing costs are described above.