Category Archives: Dairy Research

Fluid milk as a model system for development of data-informed approaches to reducing food waste

As part of a current project at Cornell University, supported by the Foundation for Food and Agriculture Research (FFAR), we organized a stakeholder webinar on development of data-informed approaches to reducing food waste from primary production to consumers. The opening presentation was presented by Martin Wiedmann, the Gellert Family Professor in Food Safety, where he first covered the basics of food waste and food loss. For example, we found out that food loss and food waste are often used interchangeably, however food waste is specifically used to describe decrease in food quality and quantity due to actions and decisions made by retailers, service providers and consumers. While food loss is describing decrease in quality and quantity of food due to actions and decisions of food suppliers earlier in the chain, both food loss and food waste are greatly impacted by shelf-life of the food. Martin continued his presentation by using fluid milk as a specific example of (i) how to develop predictive models that can be used as a decision support tools to reduce waste and loss of fluid milk from grass-to-glass, and (ii) how to develop new approaches to dynamically predict product shelf-life and price.

There are two general groups of culprits responsible for spoilage of fluid milk, (i) different cold-tolerant Gram-negative bacteria, like different Pseudomonas species and coliforms, and (ii) different cold-tolerant spore-forming bacteria, like Bacillus weihenstephanensis and different Paenibacillus species. Frist group of microorganisms are generally introduced into milk as post-processing contamination while the sporeforming bacteria are typically introduced with raw milk and can survive the pasteurization process. The first presented model was developed to predict spoilage of pasteurized fluid milk due to growth of cold-tolerant sporeforming bacteria and its design followed four specific steps as part of the simulation: (i) it selects a raw bulk tank spore concentration based on concentrations that were previously determined in raw milk from different dairy farms, (ii) it selects a single sporeformer subtype that will spoil the milk based on the subtypes identified in raw milk from these dairy farms, (iii) it applies specific growth parameters for that subtype determined in laboratory growth experiments, and finally (iv) it calculates bacterial counts in milk at different days of shelf-life. The model repeats these four steps thousands of times to give us the final range of results and estimation of how confident we are in these results. A similar model was presented that predicts spoilage of pasteurized fluid milk due to post-processing contamination with cold-tolerant Gram-negative bacteria and there was also a promise of a complete model that takes into account all of the culprits responsible for spoilage of fluid milk. One glance of the future, that was presented and where these models could be used together with other digital solutions, was a container of milk equipped with a time/temperature sensor and a QR-code that would connect to a predictive model that accounts for both inherent characteristics of that specific container of milk that are related to raw milk and processing conditions used to make it as well as the time/temperature regimes it was subjected to during transport and storage. The model like this could not only give information about the shelf-life left on this specific product but also adjust the price to incentivize a purchase of older product and reduce the product waste.

The dairy predictive models (i.e. fluid milk, yogurt) as well as some produce predictive models that were presented were mostly developed based on large sets of data collected from across the state and country; however, if the data is available these models can be made specific to a single facility or a company to create something that is known as a ‘’Digital Twin’’. A ‘’Digital Twin’’ of a food processing facility captures all of the specific characteristics of that facility and as such it can function in this digital format the same way this facility functions in a real world. What this ‘’Digital Twin’’ offers to a facility or a company is a tool to test different ‘’What-If’’ scenarios, including some scenarios that would in real world require large investments or result in large losses. For example, there are number of different interventions available that can potentially be used to extend shelf-life of fluid milk. A ‘’Digital Twin’’ can help you evaluate these interventions from both cost and benefit perspective to make the most optimal decision for your product and your company.

It looks like Cornell is well on its way to develop these and other tools for the food industry. Examples presented during this webinar are relevant to the dairy and produce industry; however, the same principles can be also applied to develop tools for other commodities and other food industry needs. Identifying these needs and developing tools that will in the end prove useful to the food industry is why this type of stakeholder engagements and exchange of information are so important for the success of digital innovations in the future.

Feel free to reach out to us at at543@cornell.edu with any questions, comments, or interests for collaboration. You can also check out our new webpage dedicated to Digital Dairy where you can find all of the information on our work in this field including the recording and presentation slides from our stakeholder webinar.

 

Recent Publications September 2021

Arora, Bindvi, and Syed S H Rizvi. “Process Optimisation and Product Characterisation of Milk Protein Concentrate Extrudates Expanded by Supercritical Carbon Dioxide.” International Journal of Dairy Technology, July 5, 2021, 1. https://doi.org/10.1111/1471-0307.12801.

Flynn, Brenna, Dana deRiancho, Marie R. Lawton, and Samuel D. Alcaine. “Evaluation of Lactose Oxidase as an Enzyme-Based Antimicrobial for Control of L. Monocytogenes in Fresh Cheese.” Foods 10, no. 7 (July 2021): 1471. https://doi.org/10.3390/foods10071471.

“Frontiers | Optimizing Pasteurized Fluid Milk Shelf-Life Through Microbial Spoilage Reduction | Sustainable Food Systems.” Accessed September 1, 2021. https://www.frontiersin.org/articles/10.3389/fsufs.2021.670029/full.

Ganda, Erika, Kristen L Beck, Niina Haiminen, Justin D Silverman, Ban Kawas, Brittany D Cronk, Renee R Anderson, Laura B Goodman, and Martin Wiedmann. “DNA Extraction and Host Depletion Methods Significantly Impact and Potentially Bias Bacterial Detection in a Biological Fluid.” MSystems 6, no. 3 (June 29, 2021): e0061921. https://doi.org/10.1128/mSystems.00619-21.

Liao, J., X. Guo, D.L. Weller, S. Pollak, D.H. Buckley, M. Wiedmann, and O.X. Cordero. “Nationwide Genomic Atlas of Soil-Dwelling Listeria Reveals Effects of Selection and Population Ecology on Pangenome Evolution.” Nature Microbiology, 2021. https://doi.org/10.1038/s41564-021-00935-7.

Portnoy, M., C. Coon, and D. M. Barbano. “Performance Evaluation of an Enzymatic Spectrophotometric Method for Milk Urea Nitrogen.” Journal of Dairy Science, August 11, 2021. https://doi.org/10.3168/jds.2021-20308.

Vogel, Kenneth G., B. G. Carter, N. Cheng, D. M. Barbano, and M. A. Drake. “Ready-to-Drink Protein Beverages: Effects of Milk Protein Concentration and Type on Flavor.” Journal of Dairy Science, July 22, 2021, S0022-0302(21)00761-X. https://doi.org/10.3168/jds.2021-20522.

Weller, Daniel Lowell, Tanzy M. T. Love, and Martin Wiedmann. “Comparison of Resampling Algorithms to Address Class Imbalance When Developing Machine Learning Models to Predict Foodborne Pathogen Presence in Agricultural Water.” Frontiers in Environmental Science 0 (2021). https://doi.org/10.3389/fenvs.2021.701288.

 

Recent Publications July 2021

Cornell Chronicle. “All in the Family: Cornell Adds Five Species to Listeria Genus.” Accessed May 21, 2021. https://news.cornell.edu/stories/2021/05/all-family-cornell-adds-five-species-listeria-genus.

Carter, Brandon, Larissa DiMarzo, Joice Pranata, David M. Barbano, and MaryAnne Drake. “Efficiency of Removal of Whey Protein from Sweet Whey Using Polymeric Microfiltration Membranes.” Journal of Dairy Science, January 1, 2021. https://doi.org/10.3168/jds.2020-18771.

“Frontiers | Moving Past Species Classifications for Risk-Based Approaches to Food Safety: Salmonella as a Case Study | Sustainable Food Systems.” Accessed June 15, 2021. https://www.frontiersin.org/articles/10.3389/fsufs.2021.652132/full.

Marcus, Justin Fisk, Timothy A. DeMarsh, and Samuel David Alcaine. “Upcycling of Whey Permeate through Yeast- and Mold-Driven Fermentations under Anoxic and Oxic Conditions.” Fermentation 7, no. 16 (January 1, 2021): 16. https://doi.org/10.3390/fermentation7010016.

Nielsen, Line, Maria Rolighed, Ariel Buehler, Susanne Knøchel, Martin Wiedmann, and Cecilie Marvig. “Development of Predictive Models Evaluating the Spoilage-Delaying Effect of a Bioprotective Culture on Different Yeast Species in Yogurt.” Journal of Dairy Science, June 11, 2021, S0022-0302(21)00669-X. https://doi.org/10.3168/jds.2020-20076.

Orsi, Renato H., Balamurugan Jagadeesan, Leen Baert, and Martin Wiedmann. “Identification of Closely Related Listeria Monocytogenes Isolates with No Apparent Evidence for a Common Source or Location: A Retrospective Whole Genome Sequencing Analysis.” Journal of Food Protection 84, no. 7 (July 1, 2021): 1104–13. https://doi.org/10.4315/JFP-20-417.

Torres-Frenzel, Pablo, Timothy A. DeMarsh, and Samuel D. Alcaine. “Investigation of the Surface-Application of Lactose Oxidase to Fresh Mozzarella Cheese as a Potential Means of Inhibiting Blue Discoloration.” Food Control 130 (December 1, 2021): 108289. https://doi.org/10.1016/j.foodcont.2021.108289.

 

Recent Publications May 2021

Beldie, Anamaria Andreea, and Carmen I. Moraru. “Forward Osmosis Concentration of Milk: Product Quality and Processing Considerations.” Journal of Dairy Science 0, no. 0 (April 14, 2021). https://doi.org/10.3168/jds.2020-20019.

———. “Forward Osmosis Concentration of Milk: Product Quality and Processing Considerations.” Journal of Dairy Science, April 15, 2021. https://doi.org/10.3168/jds.2020-20019.

Carter, Brandon, Larissa DiMarzo, Joice Pranata, David M. Barbano, and MaryAnne Drake. “Determination of the Efficiency of Removal of Whey Protein from Sweet Whey with Ceramic Microfiltration Membranes.” Journal of Dairy Science, April 2, 2021. https://doi.org/10.3168/jds.2020-18698.

Di Marzo, Larissa, Joice Pranata, and David M. Barbano. “Measurement of Casein in Milk by Kjeldahl and Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis.” Journal of Dairy Science, April 2, 2021. https://doi.org/10.3168/jds.2020-18794.

Orsi, Renato H., Soraya Chaturongakul, Haley F. Oliver, Lalit Ponnala, Ahmed Gaballa, and Martin Wiedmann. “Alternative σ Factors Regulate Overlapping as Well as Distinct Stress Response and Metabolic Functions in Listeria Monocytogenes under Stationary Phase Stress Condition.” Pathogens 10, no. 4 (April 2021): 411. https://doi.org/10.3390/pathogens10040411.

Portnoy, M., C. Coon, and D. M. Barbano. “Infrared Milk Analyzers: Milk Urea Nitrogen Calibration.” Journal of Dairy Science, April 2, 2021. https://doi.org/10.3168/jds.2020-18772.

Portnoy, Matilde, and David M. Barbano. “Lactose: Use, Measurement, and Expression of Results.” Journal of Dairy Science, April 1, 2021. https://doi.org/10.3168/jds.2020-18706.

 

Milk Quality Improvement Program publishes recommendations for enumerating bacterial spores in dairy powder

By Nicole Martin

Bacterial endospores, or spores, are an important microbial markers in many milk powders, especially in products that will be used in certain products like infant formula. There is no single standard method for enumerating spores in dairy powder that is used across the industry, which can lead to large differences in the spore test results. The basis of spore testing is similar for all methods, a sample of milk powder is rehydrated, heat treated to eliminate all bacterial cells with the exception of spores, plated on microbiological media and then incubated before enumeration. Differences in commonly used heat treatment times and temperatures, plating methods, microbiological media and incubation temperatures ultimately lead to hundreds of possible unique spore tests being used in the dairy industry. This lack of standardization for milk powder spore testing methods leads to considerable challenges for the dairy industry, including; (i) inability to benchmark against industry spore levels; (ii) lack of comparability between laboratories, and; (iii) potential competitive disadvantage against companies using methods that result in lower spore counts. To address these issues, our team evaluated 48 different spore tests on commercial milk powders with the goal of ultimately recommending a set of spore testing methods that would reliably enumerate spores in milk powders. Our analysis identified a set of three spore test methods that were able to reliably enumerate spores in commercial dairy powders. These methods include:

  • Mesophilic Spore Count: 80°C/12 min heat treatment followed by spread plating on Plate Count Milk Agar (PCMA) and incubated at 32°C for 48 h
  • Highly Heat Resistant Thermophilic Spore Count: 100°C/30 min heat treatment followed by spread plating on PCMA and incubated at 55°C for 48 h
  • Specially Thermoresistant Spore Enumeration: 100°C/30 min heat treatment followed by spread plating on PCMA and incubated at 55°C for 48 h

Our research described here was recently published in the Journal of Dairy Science (https://doi.org/10.3168/jds.2020-19313) and provides industry with a practical set of methods that addresses key challenges with milk powder spore testing. To learn more about spore testing in milk powders or to get a copy of this publication email Nicole Martin at nicole.martin@cornell.edu.

Interested in New Processing Technologies? Let us Know!

Is your business being approached by companies selling tempting new technologies, but your team isn’t sure if the investment is worth it? Our Milk Quality Improvement Program is assisting dairy processors with this question. We are seeking input on which emerging processing technologies are of interest to dairy processors to help evaluate the efficacy and value of these advancements.

New technologies are an important component in satisfying the demand for New York State dairy products and dairy ingredients, but these technologies need to be safe, effective, and promising for companies to invest in the implementation of these new solutions. Our Milk Quality Improvement Program will be expanding critical evaluation of the technical data and application associated with technologies of interest to dairy processors. By doing so we can provide better insight into how realistic, effective, and worthwhile these specific technologies are.

This project will benefit dairy producers and the overall dairy industry by facilitating innovation while limiting the amount of failed technology investment in the dairy processing sector. Share your thoughts with us by emailing Aljosa Trmcic at at543@cornell.edu or Rob Ralyea at rdr10@cornell.edu.

Recent Publications March 2021

Belias, Alexandra, Laura K. Strawn, Martin Wiedmann, and Daniel Weller. “Small Produce Farm Environments Can Harbor Diverse Listeria Monocytogenes and Listeria Spp. Populations.” Journal of Food Protection 84, no. 1 (January 1, 2021): 113–21. https://doi.org/10.4315/JFP-20-179.

Cheng, Rachel A., and Martin Wiedmann. “Recent Advances in Our Understanding of the Diversity and Roles of Chaperone-Usher Fimbriae in Facilitating Salmonella Host and Tissue Tropism.” Frontiers in Cellular and Infection Microbiology 10 (2021). https://doi.org/10.3389/fcimb.2020.628043.

Liao, Jingqiu, Peter Bergholz, and Martin Wiedmann. “Adjacent Terrestrial Landscapes Impact the Biogeographical Pattern of Soil Escherichia Coli Strains in Produce Fields by Modifying the Importance of Environmental Selection and Dispersal.” Applied and Environmental Microbiology 87, no. 6 (February 26, 2021). https://doi.org/10.1128/AEM.02516-20.

Ma, Yvonne, Jessica Chen, Karen Fong, Stephanie Nadya, Kevin Allen, Chad Laing, Kim Ziebell, et al. “Antibiotic Resistance in Shiga Toxigenic Escherichia Coli Isolates from Surface Waters and Sediments in a Mixed Use Urban Agricultural Landscape.” Antibiotics 10, no. 3 (March 2021): 237. https://doi.org/10.3390/antibiotics10030237.

Makki, Ghadeer, and Samuel D. Alcaine. “Evaluation of Lactose Oxidase as Enzymatic Antifungal Control for Penicillium Spoilage in Yogurt.” Journal of Dairy Science, March 6, 2021. https://doi.org/10.3168/jds.2020-19602.

Makki, Ghadeer M., Sarah M. Kozak, Katharine G. Jencarelli, and Samuel D. Alcaine. “Evaluation of the Efficacy of Commercial Protective Cultures to Inhibit Mold and Yeast in Cottage Cheese.” Journal of Dairy Science, January 15, 2021. https://doi.org/10.3168/jds.2020-19136.

Reichler, S. J., S. I. Murphy, N. H. Martin, and M. Wiedmann. “Identification, Subtyping, and Tracking of Dairy Spoilage-Associated Pseudomonas by Sequencing the IleS Gene.” Journal of Dairy Science, January 15, 2021. https://doi.org/10.3168/jds.2020-19283.

Rivera Flores, Viviana K., Timothy A. DeMarsh, and Samuel D. Alcaine. “Lactose Oxidase: Enzymatic Control of Pseudomonas to Delay Age Gelation in UHT Milk.” Journal of Dairy Science, December 25, 2020. https://doi.org/10.3168/jds.2020-19452.

Ubeyitogullari, Ali, and Syed S. H. Rizvi. “Heat Stability of Emulsions Using Functionalized Milk Protein Concentrate Generated by Supercritical Fluid Extrusion.” Food & Function 11, no. 12 (December 17, 2020): 10506–18. https://doi.org/10.1039/D0FO02271C.

Wang, Linran, and Carmen I. Moraru. “High-Pressure Structuring of Milk Protein Concentrate: Effect of PH and Calcium.” Journal of Dairy Science 104, no. 4 (April 1, 2021): 4074–83. https://doi.org/10.3168/jds.2020-19483.

———. “Structure and Shelf Stability of Milk Protein Gels Created by Pressure-Assisted Enzymatic Gelation.” Journal of Dairy Science 104, no. 4 (April 2021): 3970–79. https://doi.org/10.3168/jds.2020-19484.

Zwietering, Marcel H, Alberto Garre, Martin Wiedmann, and Robert L Buchanan. “All Food Processes Have a Residual Risk, Some Are Small, Some Very Small and Some Are Extremely Small: Zero Risk Does Not Exist.” Current Opinion in Food Science 39 (June 1, 2021): 83–92. https://doi.org/10.1016/j.cofs.2020.12.017.

 

Services to Know About in 2021

There are many services offered through our Extension Program, the Food Safety Laboratory, and the Expertise of our team that can help your organization, whether it’s to address an acute or persistent food safety or quality issues, to prepare for an audit,  to implement or improve continuous improvement or troubleshooting procedures, or to develop improved training programs for a team or for individuals that you want to grow in your organization. We can help you take things off your to-do list in 2021.

The list below encompasses many of the services we encourage businesses to take advantage of. If your organization is interested in any of the services listed below, or more specific support not outlined below, please reach out to Anika Gianforte at adz8@cornell.edu at any point, or submit a request through our website. We also encourage individuals and organizations to reach out to us with any questions or issues through our “Ask an Expert” platform. These questions will be directed to the appropriate team member. These services in combination with our workshop programming allow us to equip food businesses with knowledge and tools to flourish.

  • Lab for Molecular Typing: The lab offers a wide variety of technologies to subtype, identify, and characterize microorganisms to the genus, species, subspecies, and subtype level including Pulsed Field Gel Electrophoresis (PFGE) and Whole Genome Sequencing (WGS). Services are strictly confidential. We do not submit isolates or patterns to the CDC unless specifically requested by the submitter/customer. A considerable number of companies use our services to enhance their environmental monitoring programs or to help troubleshoot microbiological issues.
  • Pathogen Environmental Monitoring Validation Services: We are available to review or assist in the development of your pathogen environmental monitoring programs. We also validate environmental monitoring programs through plant visits with collection of samples; this will help you determine whether your program is designed and implemented correctly to identify any issues you may have; we can also design these visits to represent mock FDA “swabathons”, helping you ensure your company is ready for a visit by the FDA or another regulatory agency.
  • Process Authority Review: For those who require a scheduled process to begin processing a dairy food product, our team member Rob Ralyea will review your proposed process for food safety. This process review will then be submitted to your regulatory authority for their approval. We also will refer you to other Cornell process authorities if you need help with non-dairy processes.
  • Food Safety Plan Review: Our team members are instructors of both HACCP and Preventive Controls for Human Food curricula, and are qualified to review or assist in developing your facility’s food safety plan. This service is useful for facilities who have an existing plan, are in the process of developing a new plan, or who are modifying their HACCP plan to meet the requirements of a FSMA food safety plan.
  • Gap Audit: Gap audits are used to identify areas for improvement in your food safety or quality system, and are a great way to prepare for a GFSI audit. Kimberly Bukowski, who is also an SQF auditor, visits your facility to evaluate both documentation and personnel/operational practices to help identify gaps in your systems.
  • On-site consulting: Extension personnel visit and consult with dairy processors to help troubleshoot or improve the food safety and quality of their products. Examples include visits to assess technical issues including quality, spoilage, or food safety concerns, food defense plan review, and special project consultation, and documentation review.
  • Milk Quality Improvement Program: Offers targeted fee-for-service microbiological and sensory testing to assist dairy processors with troubleshooting quality issues. Services include testing for groups of spoilage organisms, identification and subtyping, defect identification, and more.
  • Sensory Evaluation Center: Services include Consumer Acceptability, Preference, and Discrimination Testing. In addition, the program provides consultations in all matters related to sensory product testing: appropriateness of particular test methods, study sample size, questionnaire design, statistics, and analyses, sample blinding, and serving sizes, to name a few.
  • Institute for Food Safety COVID-19 Resources: This website is consistently updated with relevant resources and information for the food industry. Resources include information on upcoming industry office hours, weekly videos, templates and trainings, and updates on laws, regulations, and regulatory guidance.
  • On-farm Rapid Response Team: Offers timely assistance to NY dairy processors to troubleshoot farm related quality and safety issues  ​
  • Alcaine Research Group (ARG) Dairy Lab: Quality control, waste reduction, and new product development opportunities
  • Food Processing Development Laboratory (FPDL): The Cornell Food Processing Development Laboratory (FPDL) is a licensed dairy plant in NY State and offers a unique food product development and manufacturing space that allows individuals and companies to perform research, develop new products, and start new food related businesses. Recognizing the diverse needs of industry, we offer an opportunity to utilize this facility under four different types of arrangements (see flyer here):
    1. Product Testing: For small scale pilot research projects that can be completed in 100 days or less.
    2. Cheese and Dairy Product Incubator: Designed for companies who do not have an existing facility, the FPDL can be used for initial product development and production.
    3. Cheese and Dairy Product Incubator (Level II): Designed for companies who do not have an existing facility, but have done prior product development or for companies that have an existing facility but require an outside facility for specialized needs.
    4. Contract Manufacturing and Product Development: For small scale manufacturing and pilot production (e.g. identifying processing issues, optimizing processes prior to large scale manufacturing, samples for market research).

 

If you need help with anything else that is not listed here, please also contact us – we probably have the resources to help you.

Recent Publications: January 2021

Bach, K.D., D.M. Barbano, and J.A.A. McArt. 2020. “The Relationship of Excessive Energy Deficit with Milk Somatic Cell Score and Clinical Mastitis.” Journal of Dairy Science. https://doi.org/10.3168/jds.2020-18432.

Carroll, Laura M., Jana S. Huisman, and Martin Wiedmann. 2020. “Twentieth-Century Emergence of Antimicrobial Resistant Human- and Bovine-Associated Salmonella Enterica Serotype Typhimurium Lineages in New York State.” Scientific Reports 10 (1): 14428. https://doi.org/10.1038/s41598-020-71344-9.

Carroll, Laura M., and Martin Wiedmann. 2020. “Cereulide Synthetase Acquisition and Loss Events within the Evolutionary History of Group III Bacillus Cereus Sensu Lato Facilitate the Transition between Emetic and Diarrheal Foodborne Pathogens.” MBio 11 (4). https://doi.org/10.1128/mBio.01263-20.

“Effect of Weather on the Die-Off of Escherichia Coli and Attenuated Salmonella Enterica Serovar Typhimurium on Preharvest Leafy Greens Following Irrigation with Contaminated Water | Applied and Environmental Microbiology.” n.d. Accessed September 4, 2020. https://aem-asm-org.proxy.library.cornell.edu/content/86/17/e00899-20.

Gopirajah, Rajamanickam, Poonam Singha, Sumera Javad, and Syed S. H. Rizvi. n.d. “Emulsifying Properties of Milk Protein Concentrate Functionalized by Supercritical Fluid Extrusion.” Journal of Food Processing and Preservation n/a (n/a): e14754. https://doi.org/10.1111/jfpp.14754.

Harrand, Anna Sophia, Laura K. Strawn, Paola Mercedes Illas-Ortiz, Martin Wiedmann, and Daniel L. Weller. 2020. “Listeria Monocytogenes Prevalence Varies More within Fields Than between Fields or over Time on Conventionally Farmed New York Produce Fields.” Journal of Food Protection 83 (11): 1958–66. https://doi.org/10.4315/JFP-20-120.

Kapoor, Ragya, Apratim Jash, and Syed S. H. Rizvi. 2021. “Shelf-Life Extension of Paneer by a Sequential Supercritical-CO2-Based Process.” LWT 135 (January): 110060. https://doi.org/10.1016/j.lwt.2020.110060.

Lee, Michelle C., Younas Dadmohammadi, Chen Tan, and Alireza Abbaspourrad. 2020. “Mitigating the Astringency of Acidified Whey Protein in Proteinaceous High Internal Phase Emulsions.” ACS Applied Bio Materials, November. https://doi.org/10.1021/acsabm.0c00767.

Makki, Ghadeer M., Sarah M. Kozak, Katharine G. Jencarelli, and Samuel D. Alcaine. 2020a. “Evaluation of the Efficacy of Commercial Protective Cultures against Mold and Yeast in Queso Fresco.” Journal of Dairy Science, September. https://doi.org/10.3168/jds.2020-18769.

———. 2020b. “Evaluation of the Efficacy of Commercial Protective Cultures against Mold and Yeast in Queso Fresco.” Journal of Dairy Science 0 (0). https://doi.org/10.3168/jds.2020-18769.

Martin, N. H., P. Torres-Frenzel, and M. Wiedmann. 2020. “Invited Review: Controlling Dairy Product Spoilage to Reduce Food Loss and Waste.” Journal of Dairy Science, December. https://doi.org/10.3168/jds.2020-19130.

Reichler, S. J., S. I. Murphy, A. W. Erickson, N. H. Martin, A. B. Snyder, and M. Wiedmann. 2020. “Interventions Designed to Control Postpasteurization Contamination in High-Temperature, Short-Time-Pasteurized Fluid Milk Processing Facilities: A Case Study on the Effect of Employee Training, Clean-in-Place Chemical Modification, and Preventive Maintenance Programs.” Journal of Dairy Science 103 (8): 7569–84. https://doi.org/10.3168/jds.2020-18186.

Skeens, Jordan W., Martin Wiedmann, and Nicole H. Martin. 2020. “Spore-Forming Bacteria Associated with Dairy Powders Can Be Found in Bacteriological Grade Agar–Agar Supply.” Journal of Food Protection 83 (12): 2074–79. https://doi.org/10.4315/JFP-20-195.

Ubeyitogullari, Ali, and Syed S. H. Rizvi. 2020. “Production of High-Purity Phospholipid Concentrate from Buttermilk Powder Using Ethanol-Modified Supercritical Carbon Dioxide.” Journal of Dairy Science, August. https://doi.org/10.3168/jds.2020-18697.

Yoon, Ashton K., and Syed S. H. Rizvi. 2020. “Functional, Textural, and Sensory Properties of Milk Protein Concentrate-Based Supercritical Fluid Extrudates Made with Acid Whey.” International Journal of Food Properties 23 (1): 708–21. https://doi.org/10.1080/10942912.2020.1753768.

 

Detecting fluid milk post-pasteurization contamination using our modified Coliform Petrifilm method

By Nicole Martin, PhD

Post-pasteurization contamination of fluid milk is a major contributor to premature spoilage. The microorganisms often responsible for this contamination are Pseudomonas and other Gram-negative bacteria. When introduced into fluid milk these Gram-negative bacteria grow rapidly at refrigeration temperatures and cause off-odors and flavors as well as body defect such as coagulation. In order to reduce post-pasteurization contamination, and thereby premature product spoilage, fluid milk processors need to be able to detect Gram-negative bacteria in fluid milk products. The standard method to detect Gram-negative bacteria in fluid milk uses Crystal Violet Tetrazolium Agar (CVTA), a traditional microbiological agar. While this method has been shown to detect Pseudomonas and other relevant Gram-negative bacteria causing post-pasteurization contamination in fluid milk, it is not accessible to many processors, especially those without the capabilities to make sterilized microbiological media in-house. Recently, the Milk Quality Improvement Program investigated the use of a modified Coliform Petrifilm method to detect Gram-negative bacteria in fluid milk. The results of this study “Short communication: Coliform Petrifilm as an alternative method for detecting total gram-negative bacteria in fluid milk” were published in the Journal of Dairy Science and showed that using Coliform Petrifilm incubated at 32°C for 48h prior to evaluation for any colony formation is an acceptable alternative to using CVTA to detect Gram-negative bacteria in fluid milk. This method is easily adoptable as many fluid milk processors already use Coliform Petrifilm and will allow for better detection of post-pasteurization contamination. Processors who wish to implement this method can read the full paper by contacting Nicole Martin at  nhw6@cornell.edu.