Multi-locus sequence analysis (MLSA) provides useful information for the taxonomic classification of Streptomyces. The common multi-locus sequence typing (MLST) scheme for Streptomyces includes the five housekeeping genes: atpD, gyrB, recA, rpoB, trpB. The file below includes aligned and concatenated sequences for these five genes [atpD (496 bp), gyrB (450 bp), recA (504 bp), rpoB (543 bp), trpB (571 bp)] representing 1,875 Streptomyces strains.

5 Gene MLST

Metadata for all listed strains can be found in the spreadsheet below:

Metadata File

Additional taxonomically informative loci from Streptomyces can be found in the table below. These sequences were previously hosted by the US Agricultural Research Service (ARS) and maintained by Dr. David Labeda. Each FASTA file contains all unaligned DNA sequences available for Streptomyces for each of the genes listed below.

atpD	rpsA	rpsI	SSU rRNA (16S)
bldB	rpsB	rpsL	trpB
dapF	rpsC	rpsQ	upP
gyrB	rpsE	rpsR1
recA	rpsF	ssgA
rpoB	rpsH	ssgB

More information on the primers and methods used to obtain these sequences can be found at the citation below.

Labeda, D. P., Dunlap, C. A., Rong, X., Huang, Y., Doroghazi, J. R., Ju, K., W. W. Metcalf (2017) Phylogenetic relationships in the family Streptomycetaceae using multi-locus sequence analysis. Antonie van Leeuwenhoek. 2017. 110:563-583. doi: 10.1007/s10482-016-0824-0