https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-024-03399-x#citeas
The study includes phenotyping infected Nicotiana benthamiana plants through excavation and machine learning assisted mapping of root traits. The modifications were correlated with the production of reactive oxygen species in the root vasculature, suggesting a strong immune response in some viral isolates, but less so in other viral isolates. Further transcriptomic profiling of the root tissue revealed that dysregulation of immune response genes, pattern recognition receptors, and signaling pathways were correlated with viral infection and symptoms in roots. This study represents a new avenue for research in GFLV, as root biology for the nematode-vectored Nepovirus have not been explored.