(Wheeler and Clayton recipe, DIS, v. 40, p. 98, 1965)
(Makes approximately 120 wide diameter glass vials)
Top-Layer:
- Special K: 10 g
- Wheat Germ: 15 g
- Kellogg’s Product 19 cereal: 5 g
- Yeast: 50 g
- Hi-Protein baby cereal: 15 g
- 1 jar (4.75oz, 135 grams) Gerbers banana baby food
- Agar: 13.5 to 14.5 g (amount varies with climate)
- Tap Water: 1 L
- 95% Ethanol: 5 mL (better to use 6.5 ml)
- Propionic acid: 5 mL (better to use 6.5 ml)
Bottom-Layer:
- Instant Drosophila medium: 1.5 cups
- Boiled Tap water: 600 mL
Cooking instructions for a stove top:
- Take out the dry cereal mix (containing all of the cereals, blended to a powder. Dry mixes are prepared ahead of time and stored at -20°C in a Ziploc bag for up to 6 months).
- In a large pot, add together 500ml of tap water and agar. Bring to a boil. Stir the agar solution CONSTANTLY to prevent agar from burning to the bottom of the pot. Cook at a boil until agar is clear. This process can take up to 30 minutes.
- Blend baby food, the dry cereal mixes, and 200 ml water in a large blender.
- Once the agar solution is clear, add in the blended wet mixture to the agar solution in to the pot. Add an additional 300 ml of tap to the pot. (Use this additional 300 ml to wash all of the cereal mix out of the blender and into the pot of agar solution.
- Lower the temperature slightly to prevent scalding.
- STIR CONSTANTLY; allow the mixture to simmer for 10 minutes.
- Carefully add in the 95% ethanol plus the propionic acid; mix thoroughly.
- Continue simmering for 5 more minutes.
- Remove from heat and pour into vials. (You do not need to let the mixture cool; it can be poured immediately after removing from heat).
Pouring Dimensions:
- Glass vials: 10 mL/vial = 1000 mL (1L)/case of 100 vials
- Plastic vials: 10 mL/vial = 1000 mL (1L)/case of 100 vials
- Glass bottles: 1/3 cup/bottle ~ 2 L/case of 24 bottles
- Plastic bottles: 1/4 cup/bottle ~ 1.5 L/case of 24 bottles
We pour Wheeler-Clayton food into wide plastic vials. Once the food has cooled to room temperature, we put folded facial tissue in along one side of the vials and then moisten it with de-ionized water. The larvae use this tissue as added surface area for pupation. Be sure to use facial tissue that has no added fragrances or colorants. Also, it helps if it is not very soft tissue.