Chemistry 6860
Physical Chemistry of Proteins
Description: Physical studies of proteins, with emphasis on using single molecule spectroscopy/microscopy/imaging and protein structure-fcuntion studies.
Topics Covered
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Physical Concepts
- Chemical structure and conformation of proteins
- Protein folding energy landscape
- Electron transfer theory
- Enzyme catalysis
- Chemical kinetics and single-molecule kinetics
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Experimental Methodologies
- Absorption & emission & Raman spectroscopy
- Fluorescence resonance energy transfer
- Confocal microscopy
- Total internal reflection fluorescence
- Single molecule spectroscopy
- Time correlated single photon counting
- Fluorescence correlation spectroscopy
- Atomic force microscopy
- Optical tweezers
- Magnetic Tweezers
- Super-resolution imaging with optical microscopy
- Electrokinetic trapping
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Protein Structure and Function
- Metalloprotein structure/function (bioinorganic chemistry)
- GFP and variants, protein labeling
- Motor proteins: myosin, dynein, kinesin, F1-ATPase
- Protein-protein interactions
- Protein-DNA interactions
- Live-cell imaging (active transport & gene expression)