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Cornell University

Cornell Biotechnology Resource Center Flow Cytometry Facility

Technical Resources for BRC Flow Cytometry Facility Users

ZOE Fluorescent Cell Imager

Quick Facts

The ZOE Fluorescent Cell Imager eliminates the complexities of cell imaging associated with traditional microscopes. This fluorescence imaging system combines the ease of use of a personal tablet with the power of an inverted microscope.

  • Simplified cell imaging — the intuitive touch-screen interface allows users to view cells, capture images, and create multichannel merges with minimal training
  • Flexible operation — brightfield and three fluorescence channels enable use for routine cell culture applications and more sophisticated imaging applications
  • Fluorescence imaging at your bench — light shield permits fluorescence imaging in ambient light
  • Large viewing area — the motorized stage and wide field of view allow you to see more of your sample, faster
  • This resource is free for all BRC users and doesn’t require reservations. 

Location: Biotech 140


Applications

Use the ZOE Cell Imager to check/screen samples prior to high-content analysis (HCA), high-throughput screening (HCS), confocal imaging, or fluorescence-activated cell sorting (FACS). With a brightfield and three fluorescent channels, the ZOE Cell Imager has all the features needed for daily cell culture work as well as fluorescent applications:

  • Visual estimation of cell confluency
  • Observation of general cell health and morphology
  • Cell growth and proliferation monitoring
  • Capturing cell images (with or without fluorescent labels)
  • Visualization of expressed fluorescent proteins
  • Immunofluorescent protein localization
  • Estimation of transfection efficiency

Instrument Configuration & Compatible Fluorophores

Blue Channel
Excitation: 355/40 nm
Emission: 433/36 nm
Green Channel
Excitation: 480/17 nm
Emission: 517/23 nm
Red Channel
Excitation: 556/20 nm
Emission: 615/61 nm
PureBlu DAPI Nuclear
Staining Dye
CytoTrack Green
511/525
ReadiLink 555/570
Antibody Labeling Kit
PureBlu Hoechst 33342
Nuclear Staining Dye
ReadiLink 492/516
Antibody Labeling Kit
ReadiLink 594/610
Antibody Labeling Kit
ReadiLink 350/440
Antibody Labeling Kit
VivaFix 498/521
Cell Viability Assay*
VivaFix 547/573
Cell Viability Assay
VivaFix 353/442
Cell Viability Assay
CFDA-SEVivaFix 583/603
Cell Viability Assay
Alexa Fluor 350Acridine OrangeAlexa Fluor 546
Alexa Fluor 405Alexa Fluor 488 dye*Alexa Fluor 568
Cascade BlueBODIPY Fl*Alexa Fluor 594
CellTracker BlueCalcein AMAlexa Fluor 610
DAPI*DiOCy3*
Hoechst*EGFP*Dil Stain
LysoTracker BlueER-Tracker GreenDsRed*
Marina BlueFITC*ER-Tracker Red
NucBlue FixedMitoTracker Green FMmCherry*
NucBlue LiveSYTO 9, SYTO 13, SYTO 16mStrawberry
SYTOX GreenMitoTracker Red*
Tubulin GreenmKate*
YFP*RFP*
SYTOX Orange
SYTO 84, SYTO 85
Texas Red*

* R&D Tested (Information obtained from Bio Rad)