Thermo Fisher Attune NxT
Quick Facts
- 4 lasers, 14 parameters*
- 405, 488, 561, 640 nm lasers
- Plate-reading capacity (96 and 384 well)
- Accepts conical, round, or flat bottom plates
* includes FSC and SSC in addition to colors
Location: Biotech 140 and Veterinary Research Tower TB-016
Technical Specifications
The Thermo Fisher Attune NxT is a compact, efficient, and flexible benchtop analyzer that utilizes acoustic-assisted hydrodynamic focusing. This enables faster sample throughput rates and minimizes clogs, and allows a broad range of cell and sample types. In addition, the Attune NxT has a positive-displacement syringe pump system for sample delivery, allowing volumetric analysis. The Attune NxT software is intuitive and easy to learn, and prompts users with step-by-step instructions for cleaning cycles and shutdown procedures. With the autosampler attachment and optional passive cooling system, the Attune can run large numbers of low volume samples in plate format.
| Excitation lasers | 405 nm, 488 nm, 561nm, 638 nm |
| Output power | 405 nm, 488 nm, 561 nm (100 mW), 638 nm (140 mW) |
| Detection parameters | 14 fluorescence + 2 scatter |
| Sample tube | Single, manual loaded tube or plate autosampler |
| Tube types | 0.5 -mL, 1.5 mL, and 5-mL tubes |
| Plate types | 96- and 384-well conical, round, or flat bottom plates |
Instrument Configuration
| Laser | Channel Label | Filter BP | Filter LP | Preferred Colors |
|---|---|---|---|---|
| 405 | VL1 | 450/50 | 410 | Brilliant Violet 421 |
| VL2 | 525/50 | 495 | Brilliant Violet 480 | |
| VL3 | 610/20 | 555 | Brilliant Violet 605 | |
| VL4 | 660/20 | Brilliant Violet 650 | ||
| VL5 | 710/50 | 680 | Brilliant Violet 711 | |
| VL6 | 780/60 | 740 | Brilliant Violet 786 | |
| 488 | BL1 | 530/30 | 503 | Alexa Fluor 488, GFP, FITC |
| BL2 | 695/40 | 555 | PerCP-Cy5.5, PerCP | |
| 561 | YL1 | 586/16 | PE | |
| YL2 | 620/15 | 577 | PE-Dazzle 594, PE-Texas Red | |
| YL3 | 780/60 | 650 | PE-Cy7 | |
| 637 | RL1 | 670/14 | 654 | APC |
| RL2 | 720/30 | 690 | Alexa Fluor 700 | |
| RL3 | 780/60 | 740 | APC-Cy7 |
Saving Your Data
Users are given access to a fileshare drive to save data after being trained on the instrument. You can save your data as experiment files, FCS files, and PDFs.
Experiment File:
1.Right-click the experiment name -> Export -> Experiment (plate experiments will say Plate)
2. Save to This PC -> FlowCyto Share X:\ drive -> Your folder -> Create new folder with today’s date.
For tube experiment, this will be .atx, for plate experiments this will be .apx file.
FCS Files:
1. Right-click the experiment name -> Export -> FCS files.
2. Save to This PC -> FlowCyto Share X:\ drive under your folder.
3. If a popup message asks if you want to Update Keywords, we do recommend selecting the
checkbox at the bottom to select all, then choose Update Keywords.
PDFs:
1. Tube experiments: Right-click the experiment name -> Print.
Plate experiments: Right-click the Group name -> Print
2. Select what workspace(s) to print -> OK -> OK
3. Save to This PC -> FlowCyto Share X:\, your folder, Save.
Don’t forget: Saving your data is your responsibility. We regularly delete experiments from our instrument’s computers to free up memory.