Skip to main content

Cornell University

Cornell Biotechnology Resource Center Flow Cytometry Facility

Technical Resources for BRC Flow Cytometry Facility Users

Supplementary Training Videos

This page contains videos created by our flow core staff consisting of FlowJo analysis demos of common experiments as well as other troubleshooting tips at the machines. Box (cloud storage) sign in using Cornell netID is needed to view videos.


Compensation and Spectral Unmixing usually do NOT need adjustments if your controls are created correctly.  Below are demonstration videos showing examples of how to adjust these matrices only because their compensation/unmixing controls were not ideal.

Compensation Adjustments in FlowJo

Manual Adjustment to Spectral Unmixing on FlowJo


FlowJo Gating Demonstration for Nuclei