Sony MA900
Quick Facts
- Biosafety level 2 cabinet
- 4 lasers, 14 parameters*
- 405, 488, 561, 640 nm lasers**
- 100 um nozzle
- Controlled sample input/output temperature
- High-speed 2-way and 4-way sorting
- High-throughput plate sorting
* includes FSC and SSC in addition to colors
** MA900 has a co-laser system (paired lasers at two different locations to illuminate different regions of samples), with blue and green lasers sharing the filters FL1-FL5, and red and violet lasers sharing the filters FL6-FL12.
Sorting Guidelines – BRC Flow Facility
Location: Biotech 140
Technical Specifications
The Sony MA900 has a micro fluidic chip-based design and advanced automation that simplifies operation. It offers a high level of automation, aseptic cleaning, QC, and sort setup to ensure optimal daily alignment of the chip to the lasers. Wizard cleaning cycles simplify maintenance. The MA900 software guides operation. A choice of eight default and five custom sort modes is available to achieve the desired purity and yield of the sorted population. Sort devices supported include 2-way and 4-way tubes as well as 6-, 12-, 48, 96-, and 384-well plates. The MA900 is installed in a custom-tailored Class II Type A2 biosafety cabinet for protection of personnel and products. The cabinet incorporates a built-in aerosol management system which operates independently to actively evacuate aerosols from the sort collection chamber. The dual routes of aerosol evacuation maximize protection. Types of sorts include high-purity 4-way sorting of a 12-color immunophenotyping panel and sorting of a 12-color immunophenotyping panel using 5 violet fluorochromes.
| Excitation lasers | 488 nm, 638 nm, 405nm, 561 nm |
| Output power | 405 nm (10 mW max), 488 nm, 638 nm, and 561 nm (36 mW max) |
| Detection parameters | 12 fluorescence + 2 scatter |
| Sample tube | Single, auto-loading tube |
| Tube types | 0.5 -mL, 1.5 mL, 5 mL, and 15-mL tubes |
| Temperature control | 5°C, 37°C |
| Agitation unit | Eccentric rotation |
| Magnetic drive | 300 rpm speed |
| Sorting chip size (nozzle) | 100 um |
| Sorting speed | 100 um (targeted) setting: 21 kHz to 23.5 kHz |
| 100 um (standard) setting: 27 kHz to 31 kHz |
Instrument Configuration
Note for panel design: Only one preferred color from each parameter can be used at a single run.
| Parameter | Colaser Group | Filter BP | Preferred colors | |
|---|---|---|---|---|
| FL 1 | 1 | 525/50 | EGFP, CFSE, FITC, AF488, SYBR Green, EYFP, mVenus, mCitrine, LIVE/DEAD GRN, Zombie Green, GFP | |
| FL 2 | 1 | 585/30 | PE, mOrange, tdTomato, DsRed/mRFP | |
| FL 3 | 1 | 627/30 | PE-Texas Red, PE-Dazzle 594, PI, LIVE/DEAD Red, Zombie Red, mCherry, AF594, mKate | |
| FL 4 | 1 | 695/50 | 7AAD, PE-Cy5, PerCP, PE-Cy5.5, PerCP-Cy5.5, PerCP-eFluor710, mPlum | |
| FL 5 | 1 | 785/60 | PE-Cy7 | |
| FL 6 | 2 | 450/50 | BV421, AF405, Zombie Violet, LIVE/DEAD Violet, DAPI, PacBlue, mCFP, BFP, Sytox Blue, Hoechst 33342 | |
| FL 7 | 2 | 525/50 | AmCyan, BV510, T-Sapphire, PacOrange, Zombie Aqua, LIVE/DEAD Aqua | |
| FL 8 | 2 | 585/30 | BV570, Zombie Yellow, LIVE/DEAD Yellow | |
| FL 9 | 2 | 617/30 | BV605 | |
| FL 10 | 2 | 665/30 | BV650, APC, CY5, AF647, LIVE/DEAD Far Red | |
| FL 11 | 2 | 720/60 | BV711, APC-Cy5.5, AF700 | |
| FL 12 | 2 | 785/60 | BV785, APC-Cy7, APC-AF750, APC-Fire 750, Zombie NIR, LIVE/DEAD NIR | |
| Laser Key: 488, 488 or 561, 561, 405, 638 (AF = Alexa Fluor, BV = Brilliant Violet) | ||||
Saving Your Data
Users are given access to a fileshare drive to save data after being trained on the instrument. You can save your data as experiment files, FCS files, and PDFs.
Exporting FCS Files:
1. Right-click the Experiment name -> Export FCS files.
2. Next to Output folder, click the three dots. Navigate to This PC -> FlowCyto X: drive -> Your Folder. Create New
Folder with today’s date. Select this new folder again, OK.
3. Export
Exporting PDFs:
1. Double-click the tube within your experiment that has saved data to open the worksheet.
2. Worksheet Tools -> Print -> Print
3. Save to This PC-> FlowCyto X: drive -> Your Folder, and give the pdf a name. Save.
Exporting Experiment:
1. File -> Database -> Export
2. Highlight your experiment from the list on the left, should be at the top if created that day.
3. Click the two arrows to bring your experiment to the list on the right
4. Next to Output File, click the three dots. Save to This PC->FlowCyto X: drive->Your folder
5. Name the experiment and include the current date!
6. Export.
7. Remove files from the software under the Experiment Tab after exported. Right-click -> Delete