Instruments
Cell Analyzers
| Analyzers | Biosafety Level | Parameters | Lasers (nm) | Sampling | Tube type* | Plate type |
|---|---|---|---|---|---|---|
| FACSymphony A3 | BSL1 | UV(6), V(6), B(4), Y(4), R(3) | 355, 405, 488, 561, 640 | Single manually loaded tube | 5 mL | Currently Unavailable |
| FACSymphony A5 SE | BSL1 | UV(10), V(14), B(10), Y(9), R(6) | 355, 405, 488, 561, 637 | Single manually loaded tube | Plate autosampler | 5 mL | 96- and 384-well plates |
| Attune NxT | BSL1 | V(6), B(2), Y(3), R(3) | 405, 488, 561, 640 | Single manually loaded tube | Plate autosampler | 0.5, 1.5, 5 mL | 96- and 384-well plates |
* The FACSymphony A3 and A5 SE only accept polystyrene tubes
Cell Sorters
| Sorters | Biosafety Level* | Parameters** | Lasers (nm) | Nozzle size | Temperature control (C) | Sorting |
|---|---|---|---|---|---|---|
| FACSMelody | BSL2* | V(3), B(4), R(2) | 405, 488, 640 | 100um | 5, 37 | 2-way | plates + tubes*** (5mL) |
| Sony MA900 | BSL2* | V(7), B(5), Y(4), R(3) | 405, 488, 561, 640 | 100um | 5, 37 | 2 and 4-way | plates + tubes (1.5, 2, 5, 15 mL) |
| FACSAria Fusion | BSL2* | V(5), B(2), Y(4), R(3) | 405, 488, 561, 640 | 70, 85, 100, and 130um | 4, 20, 37, 42 | 2 and 4-way | plates + tubes (1.5, 2, 5, 15 mL) |
| Biometrica BioSorter | BSL1 | B(3) | 488 | 500um | ambient temperature | 1-way | tube or plates (24-, 48-, 96-, or 384-well) | non-sterile |
* Biocontainment hood + aerosol management
** B: blue, R: red, UV: ultraviolet, V: violet, Y: yellow
***Polypropylene tubes