BD Biosciences FACSymphony A5 SE
Quick Facts
- 5 lasers, 48 parameters*
- Only available for high parameter analysis
- 355, 405, 488, 561, 637 nm lasers
* includes FSC and SSC in addition to colors
Location: Veterinary Research Tower TB-018
Technical Specifications
The BD Symphony A5 SE offers our highest level of simultaneous parameter detection by utilizing five lasers (including UV) and 48 detectors for maximum coverage of the fluorochrome emission spectrum. The software used by this instrument is comparable to that used by the Symphony A3 and Aria Fusion, with the additional ability for spectral unmixing.
| Excitation lasers | 355 nm, 405 nm, 488 nm, 561 nm, 637nm, |
| Output power | 405 nm (200 mW), 488 nm (150 mW) , 561 nm (150 mW), 637 nm (140 mW), 355nm (60 mW) |
| Detection parameters | 46 fluorescence + 2 scatter |
| Sample tube | Single, manual loaded tube |
| Tube types | 5.0mL polystyrene tubes |
Instrument Configuration
| Laser | Detector | Channel | Mirror | Filter | Parameter |
|---|---|---|---|---|---|
| Blue (488 nm) | A | 10 | 770 LP | 810/79 BP | B810 |
| B | 9 | 724 LP | 750/60 BP | B750 | |
| C | 8 | 685 LP | 710/50 BP | B710 | |
| D | 7 | 665 LP | 675/20 BP | B675 | |
| E | 6 | 645 LP | 660/30 BP | B660 | |
| F | 5 | 585 LP | 602/40 BP | B602 | |
| G | 4 | 570 LP | 576/20 BP | B576 | |
| H | 3 | 520 LP | 537/32 BP | B537 | |
| I | 2 | 500 LP | 510/20 BP | B510 | |
| J | 1 | – | 488/10 BP | SSC | |
| Red (637 nm) | A | 16 | 750 LP | 780/60 BP | R780 |
| B | 15 | 720 LP | 730/50 BP | R730 | |
| C | 14 | 699 LP | 710/25 BP | R710 | |
| D | 13 | 680 LP | 680/30 BP | R680 | |
| E | 12 | 665 LP | 675/20 BP | R675 | |
| F | 11 | 645 LP | 660/30 BP | R660 | |
| Violet (405 nm) | A | 28 | 810 LP | 845/70 BP | V845 |
| B | 27 | 765 LP | 785/50 BP | V785 | |
| C | 26 | 730 LP | 750/40 BP | V750 | |
| D | 25 | 690 LP | 710/40 BP | V710 | |
| E | 24 | 665 LP | 680/30 BP | V680 | |
| F | 23 | 645 LP | 660/30 BP | V660 | |
| G | 22 | 605 LP | 615/25 BP | V615 | |
| H | 21 | 585 LP | 595/30 BP | V595 | |
| I | 20 | 570 LP | 576/20 BP | V576 | |
| J | 19 | 530 LP | 540/20 BP | V540 | |
| K | 18 | 495 LP | 510/40 BP | V510 | |
| L | 17 | 465 LP | 470/15 BP | V470 | |
| M | 30 | 430 LP | 450/40 BP | V450 | |
| N | 29 | 415LP | 427/25BP | V427 | |
| UV (355 nm) | A | 40 | 765 LP | 809/82 BP | UV809 |
| B | 39 | 704 LP | 736/64 BP | UV736 | |
| C | 38 | 675 LP | 695/40 BP | UV695 | |
| D | 37 | 645 LP | 660/30 BP | UV660 | |
| E | 36 | 595 LP | 610/30 BP | UV610 | |
| F | 35 | 570 LP | 585/30 BP | UV585 | |
| G | 34 | 535 LP | 540/20 BP | UV540 | |
| H | 33 | 495 LP | 515/60 BP | UV515 | |
| I | 32 | 425 LP | 446.5/67 BP | UV446 | |
| J | 31 | 365 LP | 379/34 BP | UV379 | |
| Yellow-Green (561 nm) | A | 49 | 800 LP | 825.5/49 BP | YG825 |
| B | 48 | 750 LP | 780/60 BP | YG780 | |
| C | 47 | 735 LP | 750/40 BP | YG750 | |
| D | 46 | 699 LP | 730/50 BP | YG730 | |
| E | 45 | 680 LP | 695/40 BP | YG695 | |
| F | 44 | 665 LP | 670/20 BP | YG670 | |
| G | 43 | 645 LP | 660/30 BP | YG660 | |
| H | 42 | 595 LP | 602/40 BP | YG602 | |
| I | 41 | 570 LP | 585/30 BP | YG585 |
Saving Your Data
Users are given access to a fileshare drive to save data after being trained on the instrument. You can save your data as experiment files, FCS files, and PDFs.
Experiment File
1. Right-click experiment -> Export -> Experiment
2. Navigate to Computer -> FlowCyto X: drive -> Your Folder -> Make new
folder with today’s date
3. OK
FCS Files
1. Right-click experiment -> Export -> FCS files
2. Navigate to Computer -> FlowCyto X: drive and select the appropriate
folder
3. OK
PDFs
1. Right-click experiment -> Batch Analysis
2. Browse -> Navigate to Computer -> FlowCyto X: drive and select the
appropriate folder
3. OK
Don’t forget: Saving your data is your responsibility. We regularly delete experiments from our instrument’s computers to free up memory.