BD Biosciences FACSymphony A3
Quick Facts
- 5 lasers, 25 parameters*
- Also suitable for use for routine analysis of 2+ parameters
- 355, 405, 488, 561, 640 nm lasers
- Plate-reading capacity – Currently Unavailable
- Accepts conical, round, or flat bottom plates
* includes FSC and SSC in addition to colors
Note: Users must use Falcon tubes (Cat# 352008 or 352235 with filter cap) or Flow Cytometry tubes from MTC Bio (https://mtcbiotech.com/product/flowtubes-for-flow-cytometry-instruments/). MTC Bio strainer tubes are not as good as Falcon.
Location: Veterinary Research Tower TB-016
Technical Specifications
The Symphony A3 supports large panels with more than 20 different fluorochromes, allowing maximal information in a single experiment. The software used by this instrument is comparable to that used by the Aria Fusion, so experience translates between these instruments.
| Excitation lasers | 355 nm, 405 nm, 488 nm, 561 nm, 638nm, |
| Output power | 405 nm, 488 nm, 561 nm, 638 nm (100 mW), 355nm (20 mW) |
| Detection parameters | 23 fluorescence + 2 scatter |
| Sample tube | Single, manual loaded tube |
| Tube types | 5.0mL polystyrene |
Instrument Configuration
| Laser | Detector | Filter BP | Filter LP | Preferred Colors |
|---|---|---|---|---|
| 355 | F | 379/28 | BUV395 | |
| E | 515/30 | 450 | BUV496 | |
| D | 586/15 | 550 | BUV563 | |
| C | 670/30 | 635 | BUV661 | |
| B | 740/35 | 690 | BUV737 | |
| A | 820/60 | 770 | BUV805 | |
| 405 | F | 450/50 | 410 | AF405, BFP, BV421, Pac blue, V450 |
| E | 525/50 | 505 | AmCyan, BV480 | |
| D | 610/20 | 600 | BV570, BV605 | |
| C | 670/30 | 635 | BV650 | |
| B | 710/50 | 690 | BV711, BV750 | |
| A | 780/60 | 750 | BV786 | |
| 488 | D | 515/20 | 505 | AF488, BB515, CFSE, FITC, GFP, YFP |
| C | 610/20 | 600 | BB630-P | |
| B | 710/50 | 690 | BB700, PerCP, PerCP-Cy5.5 | |
| A | 780/60 | 770 | BB790-P | |
| 561 | D | 586/15 | 570 | AF532, AsRed2, PE, RFP |
| C | 610/20 | 600 | AF610-PE, mStrawberry, mCherry, mRaspberry, HcRed1PE-CF594, PE-CF594, PE-Texas Red | |
| B | 670/30 | 650 | 7AAD, mPlum, E2-Crimson, PE-Cy5 | |
| A | 780/60 | 750 | PE-Cy7 | |
| 628 | C | 670/30 | 650 | Alexa Fluor 647, APC |
| B | 710/50 | 690 | AF700, APC-Cy5.5, APC-R700 | |
| A | 780/60 | 750 | APC-Cy7, APC/Fire 750, APC-H7 |
Saving Your Data
Users are given access to a fileshare drive to save data after being trained on the instrument. You can save your data as experiment files, FCS files, and PDFs.
Experiment File
1. Right-click experiment -> Export -> Experiment
2. Navigate to Computer -> FlowCyto X: drive -> Your Folder -> Make new
folder with today’s date
3. OK
FCS Files
1. Right-click experiment -> Export -> FCS files
2. Navigate to Computer -> FlowCyto X: drive and select the appropriate
folder
3. OK
PDFs
1. Right-click experiment -> Batch Analysis
2. Browse -> Navigate to Computer -> FlowCyto X: drive and select the
appropriate folder
3. OK
Don’t forget: Saving your data is your responsibility. We regularly delete experiments from our instrument’s computers to free up memory.