BD Biosciences FACSAria Fusion
Quick Facts
- Biosafety level 2 cabinet
- 4 lasers, 16 parameters*
- 405, 488, 561, 640 nm lasers
- 70, 85, 100, and 130 um nozzles**
- Controlled sample input/output temperature
- High-speed 2-way and 4-way sorting
- High-throughput plate sorting
* Includes FSC and SSC in addition to colors
** Nozzle size should be 3 – 5 times larger than the particle to be sorted. The standard nozzle size in the facility is 100um as it provides a good balance of speed and range of cells.
Sorting Guidelines – BRC Flow Facility
Location: Biotech 140
Technical Specifications
The BD FACSAria Fusion is an advanced system for experienced users, achieving high sensitivity and resolution with flexibility and control. The Aria Fusion can meet a variety of needs with its range of nozzle sizes, adjustable dynamic range of sample flow rates, and selection of sort devices- including 2-way and 4-way tubes as well as 6-, 24-, 48-, 96-, and 384-well plates. The Aria Fusion is installed in a fully integrated custom Class II Type A2 biosafety cabinet for protection of personnel and products. The cabinet incorporates a built-in aerosol management system which operates independently to actively evacuate aerosols from the sort collection chamber. The dual routes of aerosol evacuation maximize protection.
| Excitation lasers | 488 nm, 640 nm, 405nm, 561 nm |
| Output power | 405 nm (>80 mW), 488 nm (>50 mW), 640 nm (>100mW), 561 nm (>50 mW) |
| Detection parameters | 14 fluorescence + 2 scatter |
| Sample tube | Single, auto-loading tube |
| Tube types | 1.5 mL, 2.0-mL, 5.0mL, and 15 mL |
| Temperature control | 4, 20, 37, or 42°C |
| Agitation unit | Eccentric rotation |
| Sorting nozzle | 70, 85, 100, and 130 um |
Instrument Configuration
| Laser | Filter BP | Filter LP | Preferred colors |
|---|---|---|---|
| 405 | 450/50 | DAPI, Brilliant Violet 421 | |
| 525/50 | 505 | Brilliant Violet 510 | |
| 610/20 | 595 | Brilliant Violet 605 | |
| 670/30 | 635 | Brilliant Violet 650 | |
| 780/60 | 750 | Brilliant Violet 786 | |
| 488 | 530/30 | 502 | FITC |
| 695/40 | 655 | PerCP-Cy5.5 | |
| 561 | 583/15 | PE | |
| 610/20 | 600 | PE-CF594 | |
| 670/14 | 630 | PE-Cy5 | |
| 780/60 | 735 | PE-Cy7 | |
| 640 | 670/30 | APC | |
| 730/45 | 690 | APC-Alexa 700 | |
| 780/60 | 755 | APC-Cy7 |
Saving Your Data
Users are given access to a fileshare drive to save data after being trained on the instrument. You can save your data as experiment files, FCS files, and PDFs.
a. R-click on the Experiment name->Batch analysis (Saves worksheet in pdf)
b. Browse to X:\FLOWCYTO\ your folder-> Make a folder for today, save to it
c. R-click on the Experiment name-> Export -> FCS files
d. Browse to X:\FLOWCYTO\your folder->Choose today’s folder, Save
e. R-click on the Experiment name->Export-> Experiment (backup file)
f. Browse to D:\BDExport\Experiment\your folder-> Export