Tips and tricks for conventional and spectral flow cytometry: How to optimize workflows to maximize biological resolution

August 13, 11am-2pm

VRT LH3 room # T1-003 or via Zoom

Spectral flow cytometry is gaining in popularity due to the potential for larger panels, but the transition from conventional cytometry can appear daunting. In this presentation, we will explore the transition from conventional to spectral cytometry and how to optimize workflows to avoid pain points. We will also dig deeper into aspects of flow cytometry that are more impactful for spectral flow cytometry, including fluorophore selection, controls, blocking buffers, and panel quality evaluation metrics. This presentation will cover topics for flow cytometrists that have experience with conventional flow cytometry as well as those that are just beginning with spectral flow cytometry.

Speaker:

Seddon Y. Thomas, Ph.D.
Staff Scientist, R&D
Protein & Cell Analysis
Thermo Fisher Scientific
Durham, North Carolina USA

Sponsored by the Institute of Biotechnology and Biotechnology Resource Center Flow Cytometry Facility

Lunch will be provided for in-person attendees

Symphony A3/A5 Practical Troubleshooting Workshop

June 24th, 1pm

VRT Basement Labs

During this 1-hour session we will demonstrate identifying and resolving common issues for both BD Symphony A3 and Symphony A5 flow cytometers. This will include pre-emptive maintenance, monitoring metrics, symptom identification, resolution of both clogs and air, and a review of provided materials and contact information. We highly encourage every interested Symphony user to attend, or at minimum to have one person from your lab or group in attendance. This is especially critical for anyone planning to use these flow cytometers during off-hours, weekends, and holidays. Moving forward we plan to hold these workshops on a semi-regular basis so that new facility users onboarded each semester can attend.

BRC Flow Cytometry: Using FluoroFinder to design experiments

Tuesday, July 23, 2024 at 12:00pm to 1:00pm

Virtual Event

Please join us for the upcoming live demo webinar on how to use the FluoroFinder platform, an essential tool for building flow cytometry panels and designing fluorescent experiments.

As a researcher at the Cornell Institute of Biotechnology, you have access to FluoroFinder’s free academic features including our Conventional and Spectral panel designers, our Spectra Viewer, our Dye Database with over 1000+ fluorophores, and our Reagent Search that indexes over 3,000,000 antibody products across 70+ vendors. Additionally, Cornell and Weill Cornell users have access to FluoroFinder’s Academic Plus features, which comes with our full flow cytometry training course and IntelliPanel, our automated panel design software. Join the webinar to learn how to take advantage of the platform or email support@fluorofinder.com with any questions.

Dial-In Information

https://register.gotowebinar.com/register/7808824379128186198