Article: Lee, TT; Garcia, JR; Paez, JI; Singh, A; Phelps, EA; Weis, S; Shafiq, Z; Shekaran, A; del Campo, A; Garcia, AJ; (2015) “Light-triggered In Vivo Activation of Adhesive Peptides Regulates Cell Adhesion, Inflammation and Vascularization of Biomaterials”, Nature Materials, 14(3):352-360
Abstract: Materials engineered to elicit targeted cellular responses in regenerative medicine must display bioligands with precise spatial and temporal control. Although materials with temporally regulated presentation of bioadhesive ligands using external triggers, such as light and electric fields, have recently been realized for cells in culture, the impact of in vivo temporal ligand presentation on cell-material responses is unknown. Here, we present a general strategy to temporally and spatially control the in vivo presentation of bioligands using cell-adhesive peptides with a protecting group that can be easily removed via transdermal light exposure to render the peptide fully active. We demonstrate that non-invasive, transdermal time-regulated activation of cell-adhesive RGD peptide on implanted biomaterials regulates in vivo cell adhesion, inflammation, fibrous encapsulation, and vascularization of the material. This work shows that triggered in vivo presentation of bioligands can be harnessed to direct tissue reparative responses associated with implanted biomaterials.
Funding Acknowledgement:
Materials World Network Program [DFG AOBJ 569628, NSFDMR-0909002]; National Institutes of Health (NIH) [R01-AR062368, R01-AR062920]; Cell and Tissue Engineering NIH Biotechnology Training Grant [T32 GM-008433]
Funding Text:
This work was supported by the Materials World Network Program grants DFG AOBJ 569628 (AdC) and NSFDMR-0909002 (A.J.G.) and the National Institutes of Health (NIH) grants R01-AR062368 and R01-AR062920 (A.J.G.). T.T.L. and J.R.G. were supported by the Cell and Tissue Engineering NIH Biotechnology Training Grant (T32 GM-008433).