Skip to main content
  Cornell University

MAE Publications and Papers

Sibley School of Mechanical and Aerospace Engineering

New article: Cell-Laden Poly (epsilion-caprolactone)/Alginate Hybrid Scaffolds Fabricated by and Aerosol Cross-Linking Process for Obtaining Homogeneous Cell Distribution: Fabrication, Seeding Efficiency, and Cell Proliferation and Distribution

Article: Lee HJ, Ahn SH, Bonassar LJ, Chun W, Kim GH, (2013) Cell-Laden Poly (epsilion-caprolactone)/Alginate Hybrid Scaffolds Fabricated by and Aerosol Cross-Linking Process for Obtaining Homogeneous Cell Distribution:  Fabrication, Seeding Efficiency, and Cell Proliferation and Distribution. Tissue Engineering Part C-Methods, 19 (10): 784-793

DOI

Abstract:   Generally, solid-freeform fabricated scaffolds show a controllable pore structure (pore size, porosity, pore connectivity, and permeability) and mechanical properties by using computer-aided techniques. Although the scaffolds can provide repeated and appropriate pore structures for tissue regeneration, they have a low biological activity, such as low cell-seeding efficiency and nonuniform cell density in the scaffold interior after a long culture period, due to a large pore size and completely open pores. Here we fabricated three different poly(epsilon-caprolactone) (PCL)/alginate scaffolds: (1) a rapid prototyped porous PCL scaffold coated with an alginate, (2) the same PCL scaffold coated with a mixture of alginate and cells, and (3) a multidispensed hybrid PCL/alginate scaffold embedded with cell-laden alginate struts. The three scaffolds had similar micropore structures (pore size = 430-580 mu m, porosity = 62%-68%, square pore shape).

Preosteoblast cells (MC3T3-E1) were used at the same cell density in each scaffold. By measuring cell-seeding efficiency, cell viability, and cell distribution after various periods of culturing, we sought to determine which scaffold was more appropriate for homogeneously regenerated tissues.

Leave a Reply

Your email address will not be published. Required fields are marked *

Skip to toolbar