Protein Gel Electrophoresis – High School **

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**IMPORTANT: ONLY TEACHERS WHO HAVE BEEN TRAINED IN THE IMPLEMENTATION OF THE ‘PROTEIN GEL ELECTROPHORESIS‘ LAB CAN BORROW THIS KIT.

Using this kit, students separate a mixture of proteins from skeletal muscle using SDS polyacrylamide gels. While analyzing and comparing protein bands according to their molecular weights on the gel, they try to establish evolutionary relatedness among different groups.

This kit includes:

  • 2 vertical gel boxes with tray inserts and stands
  • 1 power supply
  • 1 dry bath heater
  • 1 thermometer
  • 4 staining trays
  • Bottles Tris-SDS solubilization buffer (Laemmli buffer)
  • Packages Tris-Gly-SDS running buffer
  • Bottles protein fixative/stain
  • Bag of 15 mL test tubes
  • 4 test tube racks
  • 4 microfuge tube racks
  • Bag of 1.6 mL microfuge tubes
  • 1 buffer dam
  • 4 pipettes
  • 2 boxes of P20 tips
  • 1 loading guide
  • 4 plastic stirring rods
  • 1 lab notebook
  • 1 polyacrylamide gel per 4 students
  • 1 vial high molecular weight markers (100 µL per 4 gels)

Teachers must supply:

  • Various meats
  • Disposable gloves
  • Safety goggles
  • Markers
  • Tape
  • Razor blades or scalpels
  • 500 mL or 1 L flask or beaker
  • Plastic cutting boards
  • Clinical centrifuge, or funnel with cheesecloth

Protein Gel Electrophoresis - High School (ONLY  TRAINED TEACHERS CAN RESERVE THIS KIT)